Prostaglandin-Synthesizing System in Rat Liver
نویسندگان
چکیده
منابع مشابه
Synthesis of rat liver albumin in a rabbit reticulocyte cell-free protein-synthesizing system.
Rat liver polysomal RNA can direct the synthesis of albumin in a cell-free system derived from rabbit reticulocytes. The in uifro product, labeled with [3H]leucine, was identified as albumin by precipitation with specific antibody and by comparison to authentic serum [Wlalbumin. The in uifro product and serum albumin co-migrate during electrophoresis in sodium dodecyl sulfate polyacrylamide gel...
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The phosphatidate-synthesizing system of rat-liver microsomes was resolved into two component enzymes, glycerolphosphate acyltransferase and 1-acylglycerolphosphate acyltransferase. The resolution is effected by sucrose density gradient centrifugation in the presence of a nonionic detergent, Triton X-100. Combination of both enzymes results in reconstitution of the phosphatidate-synthesizing sy...
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OBJECTIVE To investigate the expression level of prostaglandins (PGs) and their de novo synthesis in dry eye (DE) disease. DESIGN Cross-sectional case-control study and in vivo mouse experimental study. PARTICIPANTS Forty-six eyes from 23 DE patients and 33 eyes from 17 age- and sex-matched controls were studied. Also, DE-induced murine eyes were compared with control eyes. METHODS Patien...
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Rat liver polysomal RNA was translated in the rabbit reticulocyte lysate and in the wheat germ cell-free protein-synthesizing systems, using [(35)S]methionine as label. The catalase (hydrogen-peroxide:hydrogen-peroxide oxidoreductase, EC 1.11.1.6) that was synthesized was isolated by immunoprecipitation and characterized by electrophoresis in sodium dodecyl sulfate/polyacrylamide gels followed ...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1978
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1978.tb12623.x